4WYZ

The crystal structure of the A109G mutant of RNase A in complex with 3'UMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-03-07
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	76.487, 51.841, 57.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.775 - 1.449
R-factor	0.1295
Rwork	0.127
R-free	0.17050
RMSD bond length	0.010
RMSD bond angle	1.445
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.449
Number of reflections	39580
<I/σ(I)>	52821
Completeness [%]	93.1
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	MIB buffer, PEG 1500