4WYM
Structural basis of HIV-1 capsid recognition by CPSF6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 134.790, 136.030, 207.257 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.468 - 2.600 |
R-factor | 0.2194 |
Rwork | 0.219 |
R-free | 0.25920 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3h4e |
RMSD bond length | 0.003 |
RMSD bond angle | 0.564 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.470 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.863 | |
Number of reflections | 117525 | |
<I/σ(I)> | 20.2 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.8 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M sodium formate, ammonium acetate, tri-sodium citrate, sodium/potassium tartrate, sodium oxamate; 0.1 M sodium HEPES, MOPS; 30% glycerol, PEG 4000 |