4WXF
Crystal structure of L-Serine Hydroxymethyltransferase in complex with glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-02-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 115.470, 115.470, 191.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.740 - 2.400 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wxb |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.881 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.159 | 0.159 |
| Number of reflections | 49080 | 6122 |
| <I/σ(I)> | 16.6 | 2.4 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 14.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | HEPES 20 mM, pH 7,5 Sodium citrate 0.85 M Cacodylate 0.1 M, pH 6,5 |
