4WWW
Crystal structure of the E. coli ribosome bound to CEM-101
This is a non-PDB format compatible entry.
Replaces: 4V80Replaces: 1VT2Replaces: 3OR9Replaces: 3ORAReplaces: 3ORBExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.759, 433.272, 618.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.783 - 3.100 |
| R-factor | 0.201089122939 |
| Rwork | 0.200 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i1m 3i1n 3i1o 3i1p |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 69.783 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.111 |
| Number of reflections | 1034420 |
| <I/σ(I)> | 8.27 |
| Completeness [%] | 97.8 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD |
