4WWC
Crystal structure of full length YvoA in complex with palindromic operator DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.979, 79.979, 239.963 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.277 - 2.903 |
| R-factor | 0.2336 |
| Rwork | 0.230 |
| R-free | 0.27870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DNA-binding and effector-binding domains of YvoA (2WV0) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.466 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.280 | 3.007 |
| High resolution limit [Å] | 2.903 | 2.903 |
| Rmerge | 0.099 | 1.979 |
| Number of reflections | 18046 | |
| <I/σ(I)> | 16.77 | 0.99 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292.15 | 0.1 M Bis-Tris pH 6.5, 25 % (w/v) PEG 3350 |
