4WWA

Crystal structure of binary complex Bud32-Cgi121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-09-30
Detector	PSI PILATUS 6M
Wavelength(s)	0.979180
Spacegroup name	I 41
Unit cell lengths	111.540, 111.540, 86.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.200 - 2.953
R-factor	0.221
Rwork	0.217
R-free	0.29590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.057
Data reduction software	XDS (2011)
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.200	3.059
High resolution limit [Å]	2.950	2.953
Number of reflections	11205
<I/σ(I)>	14.32
Completeness [%]	99.8
Redundancy	4.58

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1 M Sodium Acetate pH 4.6, 2.0 M Ammounium Sulfate, 0.1 M NaCl and 10 mM Tris-HCl pH 7.5