4WVF

Crystal structure of KPT276 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2013-10-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97924
Spacegroup name	P 43 21 2
Unit cell lengths	105.909, 105.909, 305.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.139
Rwork	0.137
R-free	0.18350
RMSD bond length	0.007
RMSD bond angle	1.183
Data reduction software	HKL-2000
Data scaling software	d*TREK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.068	0.039
Total number of observations	1188877
Number of reflections	159687
<I/σ(I)>	9.7
Completeness [%]	99.6	98.1	96.3
Redundancy	7.4	7.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	0.1M Bis-Tris pH 6.6, 0.2M NH4NO3 and 18% PEG3350