4WV5
HEAT SHOCK PROTEIN 70 SUBSTRATE BINDING DOMAIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 44.738, 52.597, 73.246 |
Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
Resolution | 21.540 - 2.040 |
R-factor | 0.1887 |
Rwork | 0.187 |
R-free | 0.22390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wv7 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.650 | 2.150 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.037 | 0.130 |
Number of reflections | 20760 | |
<I/σ(I)> | 19.1 | 6.3 |
Completeness [%] | 95.3 | 75.5 |
Redundancy | 3.1 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 11 mg/ml protein in 25mM Tris-HCl, 150 mM NaCl, 1 mM TCEP was mixed 1:1 with reservior solution containing 0.1 M Sodium Acetate, pH 4.5, 2M Ammonium Sulfate |