4WV1
Crystal structure of the FGFR2 D2 domain in complex with Fab 2B.1.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.087, 181.239, 94.427 |
| Unit cell angles | 90.00, 113.72, 90.00 |
Refinement procedure
| Resolution | 45.640 - 2.362 |
| R-factor | 0.2007 |
| Rwork | 0.198 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 3GRW and 3CU1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.640 | 2.470 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.489 | |
| Number of reflections | 46583 | |
| <I/σ(I)> | 14.4 | 1.9 |
| Completeness [%] | 98.0 | 99.3 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | PEG 3350, potassium nitrate |
