4WV1

Crystal structure of the FGFR2 D2 domain in complex with Fab 2B.1.3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	76.087, 181.239, 94.427
Unit cell angles	90.00, 113.72, 90.00

Refinement procedure

Resolution	45.640 - 2.362
R-factor	0.2007
Rwork	0.198
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3GRW and 3CU1
RMSD bond length	0.009
RMSD bond angle	1.212
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.640	2.470
High resolution limit [Å]	2.360	2.360
Rmerge		0.489
Number of reflections	46583
<I/σ(I)>	14.4	1.9
Completeness [%]	98.0	99.3
Redundancy	2.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	PEG 3350, potassium nitrate