4WUZ
Crystal structure of lambda exonuclease in complex with DNA and Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2010-03-04 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 65 |
| Unit cell lengths | 80.038, 80.038, 241.596 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.340 - 2.380 |
| R-factor | 0.23894 |
| Rwork | 0.235 |
| R-free | 0.31934 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.751 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.340 | 2.510 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.187 | 0.644 |
| Number of reflections | 33237 | |
| <I/σ(I)> | 6.5 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.5 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | 23.4% PEG 3350, 0.3M sodium acetate, 0.1 M Tris |
