4WRO
Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the second position in the A-site
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.400, 447.700, 619.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 151.956 - 3.050 |
Rwork | 0.193 |
R-free | 0.23070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3TVF 3TVE |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 200.000 | 3.150 | |
High resolution limit [Å] | 3.050 | 100.000 | 3.050 |
Rmerge | 0.325 | 0.029 | 4.566 |
Rmeas | 0.327 | 0.034 | 4.629 |
Total number of observations | 69639588 | ||
Number of reflections | 1092032 | 11 | 100022 |
<I/σ(I)> | 14.16 | 20.25 | 0.97 |
Completeness [%] | 99.9 | 23.9 | 99.9 |
Redundancy | 63.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | Tris-acetate, KSCN, PEG 550MME, PEG 20K |