4WQT
Thermus thermophilus RNA polymerase complexed with an RNA cleavage stimulating factor (a GreA/Gfh1 chimeric protein)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 189.568, 263.770, 195.848 |
Unit cell angles | 90.00, 116.83, 90.00 |
Refinement procedure
Resolution | 45.790 - 4.400 |
R-factor | 0.26 |
Rwork | 0.260 |
R-free | 0.31300 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS (1.3) |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.560 |
High resolution limit [Å] | 4.400 | 4.400 |
Rmerge | 0.669 | |
Number of reflections | 104563 | |
<I/σ(I)> | 7 | 1.5 |
Completeness [%] | 97.0 | 95.1 |
Redundancy | 3.3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | PEG 6000, potassium chloride, magnesium chloride, HEPES-NaOH |