4WQK
Crystal structure of aminoglycoside nucleotidylyltransferase ANT(2")-Ia, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-05-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.528, 42.110, 47.702 |
| Unit cell angles | 90.00, 105.49, 90.00 |
Refinement procedure
| Resolution | 37.066 - 1.482 |
| R-factor | 0.1613 |
| Rwork | 0.159 |
| R-free | 0.19590 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (phenix.autosol) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.251 | |
| Number of reflections | 28211 | |
| <I/σ(I)> | 63.68 | 5.39 |
| Completeness [%] | 96.8 | 77.4 |
| Redundancy | 7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 60 mg/mL protein, 10% 2-propanol, 20% PEG4K, 0.1 M HEPES pH 7.5 |
