4WPI
Crystal structure of USP7 ubiquitin-like domains in extended conformation
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97849 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 164.990, 164.990, 110.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.400 |
R-factor | 0.2429 |
Rwork | 0.241 |
R-free | 0.28150 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.394 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.300 |
High resolution limit [Å] | 3.400 |
Number of reflections | 21462 |
<I/σ(I)> | 24.3 |
Completeness [%] | 99.7 |
Redundancy | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | sodium chloride, TRIS-HCl, 2-mercaptoethanol, betaine hydrochloride |