4WOL
Crystal Structure of the DAP12 transmembrane domain in lipidic cubic phase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 20.775, 35.709, 101.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.669 - 1.770 |
R-factor | 0.1873 |
Rwork | 0.183 |
R-free | 0.22280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1afo |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.670 |
High resolution limit [Å] | 1.770 |
Rmerge | 0.136 |
Number of reflections | 7502 |
<I/σ(I)> | 22.26 |
Completeness [%] | 95.5 |
Redundancy | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 12.4% w/v PEG 3350, 0.149 M potassium thiocyanate |