4WOJ
Aspartate Semialdehyde Dehydrogenase from Francisella tularensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-28 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97935 |
Spacegroup name | P 1 |
Unit cell lengths | 46.587, 60.573, 67.684 |
Unit cell angles | 115.43, 100.98, 91.74 |
Refinement procedure
Resolution | 37.220 - 2.450 |
R-factor | 0.1863 |
Rwork | 0.183 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t4b |
RMSD bond length | 0.013 |
RMSD bond angle | 1.526 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.490 |
High resolution limit [Å] | 2.450 | 6.640 | 2.450 |
Rmerge | 0.054 | 0.043 | 0.201 |
Rmeas | 0.076 | 0.061 | 0.284 |
Rpim | 0.054 | 0.043 | 0.201 |
Total number of observations | 45447 | ||
Number of reflections | 23498 | ||
<I/σ(I)> | 13.7 | 3.3 | |
Completeness [%] | 97.4 | 90.7 | 94.3 |
Redundancy | 1.9 | 1.9 | 1.8 |
CC(1/2) | 0.991 | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 25% PEG3350 |