4WNE

Crystal structure of the TPR domain of LGN in complex with Frmpd4/Preso1 at 2.0 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2013-06-17
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1000
Spacegroup name	P 61 2 2
Unit cell lengths	91.784, 91.784, 176.783
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.780 - 2.000
R-factor	0.2119
Rwork	0.210
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sf4
RMSD bond length	0.009
RMSD bond angle	1.029
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (11.0.05)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.079	0.048	0.881
Total number of observations	286743
Number of reflections	30371
<I/σ(I)>	9.1		2.059
Completeness [%]	99.2	96.9	99.1
Redundancy	9.4	8.7	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.25	293	0.1 M BisTrispropane (pH 7.25), 0.2 M sodium bromide, 20% PEG 3350