4WM6

High pressure protein crystallography of hen egg white lysozyme at 950 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	298
Detector technology	CCD
Collection date	2013-02-12
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.71
Spacegroup name	P 43
Unit cell lengths	107.269, 107.269, 36.891
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.760 - 1.850
R-factor	0.2035
Rwork	0.199
R-free	0.28445
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.728
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850
Number of reflections	36471
<I/σ(I)>	19.1
Completeness [%]	99.3
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	Sodium chloride