4WM6
High pressure protein crystallography of hen egg white lysozyme at 950 MPa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2013-02-12 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.71 |
Spacegroup name | P 43 |
Unit cell lengths | 107.269, 107.269, 36.891 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.760 - 1.850 |
R-factor | 0.2035 |
Rwork | 0.199 |
R-free | 0.28445 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.728 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.850 |
Number of reflections | 36471 |
<I/σ(I)> | 19.1 |
Completeness [%] | 99.3 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | Sodium chloride |