4WLR
Crystal Structure of mUCH37-hRPN13 CTD-hUb complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.120, 99.610, 99.909 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.422 - 1.997 |
R-factor | 0.1828 |
Rwork | 0.181 |
R-free | 0.22730 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.074 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | PHENIX ((phenix.refine: dev_1760)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.420 | 38.420 | 2.050 |
High resolution limit [Å] | 2.000 | 8.930 | 2.000 |
Rmerge | 0.090 | 0.042 | 2.006 |
Rpim | 0.024 | 0.012 | 0.556 |
Total number of observations | 600550 | 6880 | 39852 |
Number of reflections | 41390 | ||
<I/σ(I)> | 17.7 | 58.7 | 1.5 |
Completeness [%] | 99.7 | 99 | 96.2 |
Redundancy | 14.5 | 12.7 | 13.7 |
CC(1/2) | 0.999 | 0.998 | 0.589 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 277 | 25% PEG 3350, 220 mM MgCl2,100 mM Bis-Tris |