4WKR
LaRP7 wrapping up the 3' hairpin of 7SK non-coding RNA (302-332)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 140 |
| Detector technology | PIXEL |
| Collection date | 2012-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.452, 33.500, 119.080 |
| Unit cell angles | 90.00, 128.99, 90.00 |
Refinement procedure
| Resolution | 59.060 - 3.200 |
| R-factor | 0.2232 |
| Rwork | 0.221 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2voo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.580 | 3.500 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 8165 | |
| <I/σ(I)> | 5.41 | 2 |
| Completeness [%] | 95.2 | 94.9 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 30% PEG 3350 & 0.1 M succinic acid, pH 7.0 |
