4WIQ
The structure of Murine alpha-Dystroglycan T190M mutant N-terminal domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 71.879, 71.879, 144.296 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.099 - 1.590 |
R-factor | 0.147 |
Rwork | 0.146 |
R-free | 0.16320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uc2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.245 |
Data reduction software | XDS (january 10, 2014) |
Data scaling software | Aimless (0.2.17) |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.100 | 1.650 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.045 | 0.442 |
Number of reflections | 35504 | |
<I/σ(I)> | 15.28 | 1.74 |
Completeness [%] | 95.0 | 71.8 |
Redundancy | 3.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | Protein solution: 5.25 mg/mL in 25 mM Tris, 150 mM NaCl and 2.5 % glycerol; pH 7.5 Precipitant solution: 0.8 M citrate buffer; pH 7.0 Drops volume: 1 microL protein solution + 1 microL precipitant solution Reservoir volume: 1 mL Crystals appeared after few days |