4WI4

Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc mutation S254A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-02
Detector	ADSC QUANTUM 210
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	49.155, 79.114, 141.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.673 - 2.800
R-factor	0.2465
Rwork	0.240
R-free	0.30790
RMSD bond length	0.017
RMSD bond angle	1.903
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Number of reflections	13759
<I/σ(I)>	24.7
Completeness [%]	96.5
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	PEG 6000, 0.1 M MES, 25% v/v ethylene glycol