4WH7
Structure of the CDC25B Phosphatase Catalytic Domain with Bound Ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-12-19 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.207, 71.442, 73.476 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.210 - 1.620 |
| R-factor | 0.1589 |
| Rwork | 0.157 |
| R-free | 0.19766 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a2k |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.187 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.154 | 0.467 |
| Number of reflections | 35426 | |
| <I/σ(I)> | 28.6 | 2.3 |
| Completeness [%] | 90.7 | 90.2 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | (NH4)2SO4, Tris, TCEP |
