4WFF
Human TRAAK K+ channel in a K+ bound nonconductive conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.666, 138.891, 96.531 |
| Unit cell angles | 90.00, 95.15, 90.00 |
Refinement procedure
| Resolution | 48.100 - 2.500 |
| R-factor | 0.21473 |
| Rwork | 0.213 |
| R-free | 0.24492 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i9w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.002 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 68997 | |
| <I/σ(I)> | 34.8 | 1 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 10.1 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 277 | 50 mM TRIS PH 8.8, 200 mM CaCl2, 27-30% (vol/vol) PEG400 |
