4WDZ
JC Polyomavirus VP1 five-fold pore mutant N221W
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 149.340, 95.980, 128.510 |
Unit cell angles | 90.00, 110.48, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.16109 |
Rwork | 0.160 |
R-free | 0.18584 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nxg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.269 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 155280 | |
<I/σ(I)> | 11.7 | 1.5 |
Completeness [%] | 98.8 | 88.5 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 0.2 M KSCN, 12% PEG 3350 |