4WD8
Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 114.079, 160.034, 161.881 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.961 - 2.300 |
R-factor | 0.1971 |
Rwork | 0.196 |
R-free | 0.21450 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.955 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 131752 |
<I/σ(I)> | 11.8 |
Completeness [%] | 99.9 |
Redundancy | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.05 M zinc acetate, 6% v/v ethylene glycol, 0.1 M sodium cacodylate, pH 6.0, 6.6 % w/v PEG 8000 |