4WCO

Crystal structure of extracellular domain of human lectin-like transcript 1 (LLT1), the ligand for natural killer receptor-P1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-18
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	82.379, 84.658, 53.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.650 - 2.460
R-factor	0.2202
Rwork	0.218
R-free	0.25720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hup
RMSD bond length	0.003
RMSD bond angle	0.776
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.700	2.550
High resolution limit [Å]	2.460	2.460
Number of reflections	14128
<I/σ(I)>	12.7	1.87
Completeness [%]	99.9	99.9
Redundancy	7.1	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M sodium cacodylate, pH6.5, 0.2 M zinc acetate and 5% (w/v) PEG8000