3HUP

High-resolution structure of the extracellular domain of human CD69

Summary for 3HUP

• Entry DOI: 10.2210/pdb3hup/pdb
• Related: 1E87 1E8I 1FM5 3CCK
• Descriptor: Early activation antigen CD69, CHLORIDE ION, SODIUM ION, ... (4 entities in total)
• Functional Keywords: c-type lectin-like domain, disulfide bond, glycoprotein, lectin, membrane, phosphoprotein, signal-anchor, transmembrane, immune system
• Biological source: Homo sapiens (human)
• Cellular location: Membrane; Single-pass type II membrane protein: Q07108
• Total number of polymer chains: 2
• Total formula weight: 30492.19

Authors

Kolenko, P.,Dohnalek, J.,Skalova, T.,Hasek, J.,Duskova, J.,Vanek, O.,Bezouska, K. (deposition date: 2009-06-15, release date: 2009-12-15, Last modification date: 2024-11-06)

Primary citation

Kolenko, P.,Skalova, T.,Vanek, O.,Stepankova, A.,Duskova, J.,Hasek, J.,Bezouska, K.,Dohnalek, J.
The high-resolution structure of the extracellular domain of human CD69 using a novel polymer
Acta Crystallogr.,Sect.F, 65:1258-1260, 2009

PubMed Abstract: The structure of the extracellular domain of human CD69 has been determined by single-crystal X-ray diffraction. The structure refined to 1.37 A resolution provides further details of the overall structure and the asymmetric interface between the monomers in the native dimer. The protein was crystallized using di[poly(ethylene glycol)] adipate, which also served as a cryoprotectant. This is the first report of a crystal structure determined using crystals grown with this polymer.

PubMed: 20054122
DOI: 10.1107/S1744309109043152

Experimental method

X-RAY DIFFRACTION (1.371 Å)