4WCG

The binding mode of Cyprinid Herpesvirus3 ORF112-Zalpha to Z-DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	P 32 2 1
Unit cell lengths	44.821, 44.821, 140.082
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.694 - 1.500
R-factor	0.179
Rwork	0.178
R-free	0.20670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hob
RMSD bond length	0.009
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.694	46.694	1.580
High resolution limit [Å]	1.500	4.740	1.500
Rmerge	0.037	0.026	0.671
Rmeas	0.040
Rpim	0.015	0.011	0.260
Total number of observations	191744	5823	28129
Number of reflections	27020
<I/σ(I)>	22.3	55.8	2.9
Completeness [%]	99.7	98.7	99.8
Redundancy	7.1	6	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293.15	0.9 M Lithium sulfate , 0.1 M HEPES pH 7.6