4WB2
Crystal structure of the mirror-image L-RNA/L-DNA aptamer NOX-D20 in complex with mouse C5a complement anaphylatoxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 46.470, 282.254, 45.899 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.877 - 1.800 |
R-factor | 0.1683 |
Rwork | 0.167 |
R-free | 0.19260 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.366 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 57409 | |
<I/σ(I)> | 21.62 | 2.98 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 0.2 M NaCl, 0.1 M Na cacodylate pH 6.0, 8% (w/v) PEG 8000 |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 0.2 M NaCl, 0.1 M Na cacodylate pH 6.0, 8% (w/v) PEG 8000 |