4WA7
Crystal Structure of a GDP-bound Q61L Oncogenic Mutant of Human GT- Pase KRas
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-12 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97924 |
Spacegroup name | P 63 |
Unit cell lengths | 82.493, 82.493, 40.761 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.721 - 1.986 |
R-factor | 0.1882 |
Rwork | 0.184 |
R-free | 0.23250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.931 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
High resolution limit [Å] | 1.980 | 5.370 | 1.980 |
Rmerge | 0.072 | 0.050 | 0.858 |
Rmeas | 0.077 | 0.054 | 0.930 |
Rpim | 0.028 | 0.020 | 0.352 |
Total number of observations | 85090 | ||
Number of reflections | 11071 | ||
<I/σ(I)> | 12.1 | ||
Completeness [%] | 99.6 | 98.7 | 96.1 |
Redundancy | 7.7 | 7.5 | 6.4 |
CC(1/2) | 0.997 | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1M MMT pH 4.0, 24% PEG6000 |