4W6C

Crystal Structure of Full-Length Split GFP Mutant K26C Disulfide Dimer, P 21 21 21 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	51.330, 88.370, 120.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.300 - 2.492
R-factor	0.2506
Rwork	0.248
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.012
RMSD bond angle	1.366
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	71.300
High resolution limit [Å]	2.490
Number of reflections	19311
<I/σ(I)>	7.28
Completeness [%]	97.3
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	35% MPD, 0.1M Imidazole pH 8.0, 0.2M MgCl2