4W5A
Complex structure of ATRX ADD bound to H3K9me3S10ph peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2012-04-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97892 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 75.522, 75.522, 137.599 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.500 - 2.600 |
R-factor | 0.224 |
Rwork | 0.221 |
R-free | 0.25970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ql9 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.800 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.096 | 0.756 |
Number of reflections | 14504 | |
<I/σ(I)> | 26.1 | 3.5 |
Completeness [%] | 99.6 | 100 |
Redundancy | 8.3 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 13-20% PEG 4000, 0.1 M MES, 3 mM MgCl2 |