4W50

Structure of the EphA4 LBD in complex with peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-03-26
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	36.270, 127.690, 84.569
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.950 - 2.420
R-factor	0.1735
Rwork	0.171
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wo1
RMSD bond length	0.011
RMSD bond angle	1.468
Data reduction software	MOSFLM
Data scaling software	Aimless (0.2.1)
Phasing software	PHASER (2.5.4)
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.950	50.950	2.520
High resolution limit [Å]	2.420	8.720	2.420
Rmerge	0.083	0.066	0.246
Rpim	0.034	0.027	0.104
Total number of observations	179142	4627	18172
Number of reflections	27280
<I/σ(I)>	14.4	23.8	4.9
Completeness [%]	93.4	99.6	90.1
Redundancy	6.6	7.1	6.3
CC(1/2)	0.995	0.989	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.2M MgCl2, 0.1M Tris pH8.5, 25% PEG3350, 4% 1,3-butanediol