4W2E

Crystal structure of Elongation Factor 4 (EF4/LepA) bound to the Thermus thermophilus 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97900
Spacegroup name	P 21 21 21
Unit cell lengths	239.290, 272.850, 431.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.760 - 2.900
R-factor	0.241
Rwork	0.238
R-free	0.30400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.634
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.940	12.930	2.970
High resolution limit [Å]	2.890	9.140	2.890
Rmerge	0.162	0.017	0.017
Number of reflections	583500	13097	34990
<I/σ(I)>	9.8	34.4	0.97
Completeness [%]	93.4	99.4	76.1
Redundancy	5.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K