4V9R

Crystal structure of antibiotic DITYROMYCIN bound to 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97900
Spacegroup name	P 21 21 21
Unit cell lengths	210.080, 449.830, 619.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.739 - 3.000
R-factor	0.2061
Rwork	0.203
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.515
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.748	3.080
High resolution limit [Å]	3.000	3.000
Rmerge	0.199	1.404
Number of reflections	1143138	84292
<I/σ(I)>	6.19	0.9
Completeness [%]	98.8	99.1
Redundancy	3.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K