4V9H
Crystal structure of the ribosome bound to elongation factor G in the guanosine triphosphatase state
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 201.580, 241.650, 305.800 |
| Unit cell angles | 90.00, 99.48, 90.00 |
Refinement procedure
| Resolution | 39.600 - 2.857 |
| R-factor | 0.22 |
| Rwork | 0.200 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.600 | 3.000 |
| High resolution limit [Å] | 2.857 | 2.857 |
| Number of reflections | 635092 | |
| <I/σ(I)> | 7.56 | 1.02 |
| Completeness [%] | 99.9 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 294 | 100mM MES pH 6.3, 75mM KCl, 6.0% PEG 20K, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 294 | 100mM MES pH 6.3, 75mM KCl, 6.0% PEG 20K, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
