4V9C
Allosteric control of the ribosome by small-molecule antibiotics
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-13 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 212.180, 433.900, 608.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.000 - 3.300 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.25500 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 3.390 | |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.182 | 0.868 |
Number of reflections | 793808 | |
<I/σ(I)> | 5.76 | 1.12 |
Completeness [%] | 95.2 | 86.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 291 | PEG800, MPD, pH 6.5, microbatch, temperature 291K | |
1 | 6.5 | 291 | PEG800, MPD, pH 6.5, microbatch, temperature 291K |