4V9A

Crystal Structure of the 70S ribosome with tetracycline.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-11
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	209.270, 448.540, 615.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	173.073 - 3.300
R-factor	0.2026
Rwork	0.202
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3UZ6 3UZ7 3UZ8 3UZ9
RMSD bond length	0.005
RMSD bond angle	0.682
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (dev_987)

Data quality characteristics

	Overall	Inner shell
Low resolution limit [Å]	300.000	300.000
High resolution limit [Å]	3.300	200.000
Rmerge	0.423	0.122
Number of reflections	859965	2
<I/σ(I)>	10.71
Completeness [%]	100.0	66.7
Redundancy	45.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K