4V8P
T.thermophila 60S ribosomal subunit in complex with initiation factor 6.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-26 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 320.190, 289.250, 535.040 |
Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
Resolution | 19.998 - 3.520 |
R-factor | 0.2163 |
Rwork | 0.216 |
R-free | 0.24450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jj2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.144 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.720 |
High resolution limit [Å] | 3.520 | 3.520 |
Rmerge | 0.130 | 0.810 |
Number of reflections | 1192534 | |
<I/σ(I)> | 8.8 | 1.6 |
Completeness [%] | 99.3 | 99.6 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |