4V8O
Crystal structure of the hybrid state of ribosome in complex with the guanosine triphosphatase release factor 3
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-27 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 204.700, 229.300, 307.000 |
| Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
| Resolution | 44.900 - 3.800 |
| R-factor | 0.293 |
| Rwork | 0.293 |
| R-free | 0.35100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2J00 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.900 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.180 | 1.400 |
| Number of reflections | 274830 | |
| <I/σ(I)> | 6.42 | 1.06 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 7.44 | 7.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 100 MM MES-KOH PH 6.7, 20 MM KCL, 7.4% (W/V) PEG20K |
