Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 208.970, 447.240, 617.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.979 - 3.100 |
| R-factor | 0.2178 |
| Rwork | 0.216 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2WDG and 2WDI |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.481 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.985 | 49.985 | 3.180 |
| High resolution limit [Å] | 3.100 | 13.860 | 3.100 |
| Rmerge | 0.228 | 0.012 | 0.012 |
| Number of reflections | 1002309 | ||
| <I/σ(I)> | 6.01 | 24.63 | 1.01 |
| Completeness [%] | 96.8 | 97.2 | 92.5 |
| Redundancy | 5.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K |
