4V8G

Crystal structure of RMF bound to the 70S ribosome.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.008
Spacegroup name	P 21 21 21
Unit cell lengths	210.240, 451.440, 621.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.748 - 3.000
R-factor	0.2198
Rwork	0.218
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.427
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.748	49.748	3.080
High resolution limit [Å]	3.000	13.420	3.000
Rmerge	0.274	0.016	0.016
Number of reflections	1142658	13178	74901
<I/σ(I)>	7.21	28.16	1.1
Completeness [%]	98.0	95	87.3
Redundancy	5.91

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K