4V84

Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-03-12
Detector	PSI PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	210.690, 451.660, 614.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.523 - 3.400
R-factor	0.2284
Rwork	0.228
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.122
Data scaling software	XSCALE
Phasing software	CNS
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.523
High resolution limit [Å]	3.400
Number of reflections	775795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	295	100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K