4V7U

Crystal structure of the E. coli ribosome bound to erythromycin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1155
Spacegroup name	P 21 21 21
Unit cell lengths	211.961, 434.529, 623.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.400 - 3.100
R-factor	0.2056
Rwork	0.205
R-free	0.25350
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3I1M 3I1N 3I1O 3I1P
RMSD bond length	0.006
RMSD bond angle	1.471
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	82.400	82.400	3.150
High resolution limit [Å]	3.100	8.410	3.100
Rmerge	0.101	0.040	0.753
Number of reflections	925719
<I/σ(I)>	10.4
Completeness [%]	83.8	98.1	78.2
Redundancy	3.3	5.4	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	4.3% PEG 8000, 4.9% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 10 mM Tris, 20 mM MES pH 6.5. 0.25 mM EDTA, microbatch, temperature 291K