4V7K
Structure of RelE nuclease bound to the 70S ribosome (postcleavage state)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-12-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 211.230, 451.430, 623.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.630 - 3.600 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.700 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 681993 | |
<I/σ(I)> | 8.39 | |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 8.47 | 7.26 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.1 M Tris-HAc, 0.2 M KSCN, 3-4.5% PEG20K (W/V), 3-4.5% PEG550 MME, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Tris-HAc | ||
2 | 1 | 1 | KSCN | ||
3 | 1 | 1 | PEG 20k | ||
4 | 1 | 1 | PEG 550 MME | ||
5 | 1 | 2 | Tris-HAc | ||
6 | 1 | 2 | KSCN | ||
7 | 1 | 2 | PEG 20k | ||
8 | 1 | 2 | PEG 550 MME |