4V6N
Structural characterization of mRNA-tRNA translocation intermediates (50S ribosome of class2 of the six classes)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | blot for 3 seconds |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.26 |
| Detector | TVIPS TEMCAM-F415 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 12.1 |
| Software | MDFF,SPIDER,Xmipp |
| Symmetry | C1 |
