4V6E
Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.952, 433.082, 624.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.436 - 3.712 |
| R-factor | 0.228 |
| Rwork | 0.227 |
| R-free | 0.26800 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.516 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.760 |
| High resolution limit [Å] | 3.700 | 10.040 | 3.700 |
| Rmerge | 0.078 | 0.039 | 0.640 |
| Number of reflections | 452913 | ||
| <I/σ(I)> | 11.4 | ||
| Completeness [%] | 75.9 | 94.2 | 41 |
| Redundancy | 2.2 | 3.4 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 291 | 4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 10 | 1 | 2 | NH4Cl | ||
| 11 | 1 | 2 | putrescine | ||
| 12 | 1 | 2 | spermidine | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | NH4Cl | ||
| 5 | 1 | 1 | putrescine | ||
| 6 | 1 | 1 | spermidine | ||
| 7 | 1 | 2 | PEG 8000 | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | MgCl2 |
