4V6B
Crystal structure of human ferritin Phe167SerfsX26 mutant.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 |
| Unit cell lengths | 212.340, 236.940, 249.710 |
| Unit cell angles | 94.69, 115.06, 114.96 |
Refinement procedure
| Resolution | 50.000 - 2.850 |
| Rwork | 0.249 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fg8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.080 | 0.322 |
| Number of reflections | 846572 | |
| <I/σ(I)> | 9.653 | 1.91 |
| Completeness [%] | 94.2 | 62.9 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM MES pH 6.5, 5% w/v PEG 6000, 2% v/v Ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
