4V5M
tRNA tranlocation on the 70S ribosome: the pre-translocational translocation intermediate TI(PRE)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 677 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.8 |
| Software | GROMACS,SPIDER |
| Symmetry | C1 |
